Cross-species amplification and characterization of microsatellite DNA loci from Gallus gallus in Bambusicola thoracica
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Graphical Abstract
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Abstract
The Chinese Bamboo Partridge (Bambusicola thoracica) is a gamebird endemic to China. Ten polymorphic microsatellite (simple sequence repeat) markers were obtained through cross-species amplification for this partridge from its relative species Gallus gallus. The number of alleles per locus varied from 4 to 13. The observed heterozygosity ranged from 0.1220 to 1.0000 and the expected heterozygosity from 0.1183 to 0.8898. Four microsatellite loci showed significant differences from the Hardy-Weinberg equilibrium. These polymorphic loci provide a valuable tool for the investigation of the phylogeography and conservation genetics of this partridge.
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